The facility houses 2 TIRF systems. One is an Olympus cellTIRF microscope system (IX 81 inverted stand) equipped with a 100x/1.45 NA Plan Apochromat TIRF objective lens (in addition to 10x, 20x, 40x, and 60x lenses), DIC & phase optics, UV, FITC, TRITC, & quad filter sets, a low-profile stage incubator (to facilitate live cell imaging), Zero Drift Compensation autofocus, an Exfo X-cite illumination system and an Andor iXon DU-897E emCCD camera. The system has 405 nm diode, Argon (458, 476, 488, 514 nm) and 561 nm diode lasers, each with independent beam steering optics for three-channel TIRF and an AOTF to regulate beam intensity.
TIRF is also available on the Nikon N-SIM structured illumination microscope. TIRF may be run independent of SIM. The microscope (Eclipse Ti inverted stand) is equipped with a CFI Apochromat TIRF 100x oil objective (NA 1.49), 'Perfect Focus' autofocus system, an Andor iXon DU-897E emCCD camera and, for TIRF, a dedicated LU-N4 laser input (405, 488, 561 & 640 nm).