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Service
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Price
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PTM Analysis 6
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Additional Sample Preparation 7
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Extended Gradient 8
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Additional Data Analysis 9
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Protein Identification (e.g., gel band, in-solution, on-bead)
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$250/sample 1
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Please Inquire
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$85/hr
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$60/hr
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$85/hr
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Label Free Quantification
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$250/sample 2
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Label Free Quantification (plasma/serum)
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$300/sample 3
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TMT-10 plex w/off-line fractionation
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$6000 4
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Bioinformatics analysis
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$125/sample 5
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1 Pricing is based on a 1 hour LC-MS/MS gradient with the instrument operated in either a data dependent or independent mode based on project goals. A gel band is defined as and excised 1D PAGE band 1-3 mm in height and a width commensurate with the gel lane.
2 Pricing includes sample total protein assay (BCA), reduction/alkylation/digestion/clean-up, total peptide determination (fluorescence), 2 hour gradient LC-MS/MS operated in data independent acquisition (DIA) mode, and Spectronaut data analysis. Minimum number of experimental replicates per condition is N = 3. Final report includes spreadsheet with identified proteins, normalized log2 expression levels, and basic statistical analysis (adjusted p-values).
3 Pricing includes everything provided in the standard LFQ analysis with additional charges related to abundant protein depletion step/reagents.
4 Pricing includes sample total protein assay (BCA), reduction/alkylation/digestion/TMT labeling/clean-up, TMT labeling efficiency QC, off-line high-pH reverse phase fractionation (10 fractions total), 2 hour gradient LC-MS/MS operated in data dependent acquisition (DIA) mode with SP3 MS3, and Proteome Discoverer data analysis. Thermo offers different levels of multiplexing for the TMT reagents so please inquire if your project requires fewer/greater number of samples. Final report includes spreadsheet with identified proteins, normalized log2 expression levels, and basic statistical analysis (adjusted p-values).
5 The VCU Bioinformatics Core has developed a data analysis pipeline for PSR LFQ datasets that includes gene enrichment, pathway analysis, heatmaps, volcano plots, and Venn diagrams. Additional pipelines are in development (e.g., PTMs) and can be discussed directly with the Bioinformatics Core Director: Dr. Jinze Liu (liuj15@vcu.edu)
6 Post-translational modification (PTM) analysis is priced based on sample, type of PTM(s), and experimental goals. In some cases, there may not be an additional charge added but in others there could be charges related to additional reagents (e.g., multiple enzymes), sample preparation time (e.g., enrichment, clean-up), and data analysis (e.g., manual spectral analysis). Please speak with lab personnel to determine which analysis type best suits your project goals.
7 Additional proteomics sample preparation is sometimes necessary. This includes enrichment, chromatographic separation, and chemical derivatization. PSR lab personnel will work with you to determine what, if any, additional sample preparation may be necessary to suit your project goals.
8 Extended gradients can provide better proteome coverage. PSR lab personnel can advise your team what, if any, extended gradient time is needed to suit your project goals.
9 Additional data analysis can be requested for datasets generated in the PSR. This may include a new search focused on PTMs, unique cleavage sites, and specialized protein database.
Massey Member Subsidy Benefit
Massey subsidizes 25 percent of the cost of members’ core laboratory usage for the following Massey-supported, fee-for-service shared resources:
- Flow Cytometry Shared Resource
- Lipidomics & Metabolomics Shared Resource
- Microscopy Shared Resource
- Tissue and Data Acquisition and Analysis Core
- Transgenic/Knockout Mouse Shared Resource
- Cancer Mouse Models Core
- Proteomics Shared Resource
